p creb p atf1 (Cell Signaling Technology Inc)
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Structured Review
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p creb p atf1
P Creb P Atf1, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 97/100, based on 2151 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/phospho+creb+ser133/Phospho-CREB+(Ser133)+Rabbit+mAb/pmc13000455-190-13-14
Average 97 stars, based on 2151 article reviews
P Creb P Atf1, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 97/100, based on 2151 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/phospho+creb+ser133/Phospho-CREB+(Ser133)+Rabbit+mAb/pmc13000455-190-13-14
Average 97 stars, based on 2151 article reviews
p creb p atf1 - by Bioz Stars,
2026-10
97/100 stars
Images
1) Product Images from "Transient SUMOylation inhibition in human pre-adipocytes stably imprints a transcriptional beiging fate"
Article Title: Transient SUMOylation inhibition in human pre-adipocytes stably imprints a transcriptional beiging fate
Journal: Nucleic Acids Research
doi: 10.1093/nar/gkag232
Figure Legend Snippet: Effect of transient SUMOylation inhibition in pre-adipocytes on cAMP-PKA-p38 signaling and adaptive thermogenesis in mature adipocytes. ( A ) Heatmap showing the stable upregulation of most genes involved in adaptive thermogenesis and cAMP-PKA-p38 signaling 22 days after adipogenic induction. Z -scores were calculated and plotted in GraphPad Prism. Treatments of pre-adipocytes were performed as shown in Fig. . ( B ) Western blot analysis of PKA substrates phosphorylation, assessed using a pan-PKA-target antibody, 22 days after adipogenic induction. TBP was used as a loading control. The asterisk (*) indicates substrates significantly affected by TAK-981 and/or rosiglitazone treatment. ( C ) Quantification of PKA substrate signals from panel (B). The signal for PKA substrates and TBP was quantified using Fiji software, with PKA substrate signals normalized to TBP. Error bars represent the standard deviation of four independent experiments. Student’s t -test was used to calculate P -values. ( D ) Western blot analysis of p-CREB, p-ATF1, p38, p-p38, and p-ATF2, 22 days after adipogenic induction. TBP was used as a loading control. ( E ) Quantification of p-CREB/ATF-1 signals from panel (D). Signals were normalized to TBP. Error bars represent the standard deviation of three independent experiments. Student’s t -test was used to calculate P -values. ( F ) Western blot analysis of p-CREB in the absence or presence of the PKA inhibitor RP-8-CPT-cAMPS. TBP was used as a loading control. Quantification of p-CREB, normalized to TBP, is showed in the lower panel. Error bars represent the standard deviation of two independent experiments. Quantification of p-p38 ( G ) and p-ATF2 ( H ) from experiment in panel (D). Signals were normalized to TBP for p-ATF2 and to p38 for p-p38. Error bars represent the standard deviation of three independent experiments. Student’s t -test was used to calculate P -values.
Techniques Used: Inhibition, Western Blot, Phospho-proteomics, Control, Software, Standard Deviation
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